Estimation of Total White Blood Cell Count
Principle: Whole blood is diluted 1 in 20 in an acid reagent which haemolyses the red cells leaving the white cells which are stained by the gential violet, (component of the diluent) to be counted. White cells are counted microscopically using an improved Neubauer ruled counting chamber and the number of WBC per liter of blood calculated.
TEST PROCEDURE FOR WHITE Blood CELL COUNT
Total White Blood Cell Count
- I Measured 0.38ml of diluting fluid and dispensed it into a tube
- 02ml of well-mixed EDTA anticoagulated venous blood was added. I assembled the counting chambers
- The diluted blood sample was re mixed using a capillary Pasteur pipette, one of the grids of the chamber was filled with the sample, taking care not to overfill the area
- Leave the chamber undisturbed for 2 minutes to allow time for white cells to settle
- The underside of the chamber was cleaned and placed on the microscope stage
- Using x 10 objective lens, I focused and counted the cells in the slide with the iris closed.
- I counted the cells in the four large corner squares of the chamber
- The number of white cells per liter of blood was counted using simple calculation as follow;
WBC count (per liter) = cells counted x 20 x 106
4 x 0.1