This is the total genetic constitution of an individual; whole blood is used to carry out the test. This tutorial will give you a good breakdown on how to get your genetic constitution.
Principle Of Genotype:
The principle behind this test is the electrophoresis on cellulose acetate paper strips which separates protein on the basis of their net charge differences and migrates towards the anode under an electric field with mobility proportional to their net charge in an alkaline medium.
- The genotype test strips are removed from the pack and immersed in 50ml of the tris-EDTA boric acid buffer of Ph 7.0 for 10 minutes.
- The buffered strips are then blotted lightly between sheets of filter paper and stretched on the bridge making sure the reference cut is positioned either in the lower right or upper left corner.
- The blood sample and known controls (usually AS) are then arranged according to their laboratory numbers on the sample application tray.
- Then press down the sample applicator tray bar to allow the sample to be absorbed, which is then transferred to the cellulose acetate strip.
- The buffered strip is put in the electrophoretic tank and the voltage is adjusted to 200 volts and time set to 20 minutes. The most common genotypes are; AA, AS, and SS.
- Results: There is no separation of protein in AA, a difference in protein leads to separation in AS, while there is no separation of protein after electrophoresis in SS.