FECAL EXAMINATION: - The simplest method for the detection of helminth egg in feaces is by direct examination of the feacal smear under the microscope using a magnification of 10 or 40. A small sample is emulsified with saline and then smeared on a slide,
which is then covered, with a slide and the whole area under examined systematically under the microscope for presence of the ova of the worm. you can also see
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In light infections concentration method are used and this is called concentration floatation method.
CONCENTRATION FLOATATION METHOD
About 4g feaces was added in a clean bottle mixed with about 12ml of water. The solution was mixed generously using a glass rod to break up the feaces sample. The solution was later centrifuged at 3,000 turns per minute for 5 minutes, thus will concentrate the ova present and the supernatant discarded.
Concentrated salt solution was added up to the brain of the tube, enabling the salt solution to float the egg in solution. About 15ml of this was collected and mounted with lactophenol cotton blue stain and glycerine, which preserves the ova. The eggs are identified and counted.
TREATMENT OF HOOKWORM
Untreated infection may persist in the individual for as long as 8 years “Alcopa” (Bephenium hydroziynaphthoate) has largely superseded all other anti-Hookworm agents including tetrachlorethylene. A single dose of alcopar dispensable granules expel Ancylostomiasis without risk; thereby halting intestinal blood loses.
Treatment with oral iron for patients of all ages will then allow normal haemoglobin level to be regulated.
Tetrachloroethylene can remove hookworm infection but Alcopar is more efficient.
The recommended dose of Alco par for all people over 2 years is single dose of 5g granules (1 Satichet). Children under 2 years 10kg in weight should receive half the above dosage. It is given on an empty stomach thereby ensuring maximum contact between parasites and drug. Food is withheld for 2 hours after the dose has been taken.
To mask the bitter taste of the drug the granules may be added to about ¼ cupful of water, milk, orange, tomato or other fruit.
Patients suffering severe Hookworm resulting in persistent diarrhea, vomiting and dehydration should have treatment withheld until the fluid balance has restored by intravenous infusion. Such patients may be giving 3 doses of 5g of Alcopar in one day in between meal. In most cases it may be necessary to continue treatment for 4-7 days to remove the infection.
HOOKWORM DIAGNOSIS AND TREATMENT