Platelets: Flow Cytometry- Platelets can be evaluated for functional defects using flow cytometry. This test uses lasers to determine proteins that are present on the
platelets surface and how they change when the platelet is activated. Platelets flow cytometry is a highly specialized procedure available only in few reference laboratories to diagnose inherited platelet function disorders
PLATELET AND DISEASE IN Flow Cytometry
High and Low Count
A normal platelet count in a healthy individual is between 150,000 and 450,000 per µl (microlitre) of blood (150-450 x 109/L). Ninety-five percent of healthy people will have platelet counts in this range. Some will have statistically abnormal platelet counts while having no demonstrable abnormality. However, if it is either very low or very high, the likelihood of an abnormality being present is higher. Both thrombocytopenia and thrombocytosis may present with coagulation problems.
In general, low platelet count increases bleeding risks; however there are exceptions. For example, immune heparin-induced thrombocytopenia and thrombocytosis (high counts) may lead to thrombosis, although this is manly when the elevated count is due to myeloproliferative disorder.
Low platelet counts are, in general, not corrected by transfusion unless the patient is bleeding or the count has fallen below 5 x 109/L. transfusion is contraindicated in Thrombotic Thrombocytopenic Purpura (TTP), as it fuels the coagulopathy.
In patients undergoing surgery, a level below 50 x 109/L is associated with abnormal surgical bleeding, and regional anesthetic procedures such as epidurals avoided for levels below 80-100.
These are widely used drugs that relieve pain and also reduce inflammation and fever. It is taken by mouth alone or in combination with other analgesics-for the relief of the less severe type of pain, such as headache, toothache, neuralgias and the pain of rheumatoid arthritis. It is also taken to reduce fever in influenza and the common cold. They work by inhibiting the production of prostaglandin; it may irritate the lining of the stomach, causing nausea, vomiting, pain and bleeding. Examples are acetylsalicylic acid (Aspirin), Vasoprine (Vasodilan), Salicylic acid, paraminophenols (Acetaminophen) etc.
- PLATELET ADHESION AND ACTIVATION, PLATELET RELEASE REACTION: Following blood vessel injury, platelets adhere to the exposed subendothelial connective tissues. Subendothelial microfibrils bind the larger multi-members of
VWF which bind to platelet membrane 1b complex.
- Platelets: BRIEF HISTORY- From antiquity to the 1800s, salicylates from plants sources (white willow, Salix alba) provided a folk remedy for pain and fever. In the mid-1800s, salicylic acid was synthesized in Europe, followed shortly thereafter by the synthesis of acetyl-salicylic.